GCUA – the Graphical Codon Usage Analyser

Differences in codon usage preference among organisms lead to a variety of problems concerning heterologous gene expression but can be overcome by rational gene design and gene synthesis. The gcua tool displays the codon quality either in codon usage frequency values or relative adaptiveness values.

Each triplet position vs. usage table
Submit a DNA sequence in raw or fasta format and choose a codon usage table. The sequence will be splitted in codons and the fraction of usage of each codon in the selected organism will be represented as one column. Additonal to the listed codon usage tables, you can submit your own by pasting in a http address.

Each codon vs. usage table
Submit a DNA sequence in raw or fasta format and choose a codon usage table. The fraction of usage of each codon in the submitted sequence will be computed and plotted against the fraction of usage of the codon in the selected organism.

Compare two usage tables
Submit or choose two codon usage tables. The fraction of usage of each codon in the submitted usage tables will be compared graphically.